Kit containing blood collection tube is sent to doctor or directly to patient. Kits contains blood collection tube, spill-proof tube carrier, pre-paid priority overnight envelope and easy to follow instructions for blood collection and shipment.
Blood collection at doctor's office or clinical lab. No centrifuging, refrigeration or other pre-shipment processing required. Sample is stable for at least 96 hrs at room temperature.
Kit box with sample is shipped to our lab in Austin, Texas using the pre-paid priority overnight envelope included in the kit. Sample pre-processing starts within hours after sample reception.
Addition of buffers, centrifugation, removal of plasma, CTC labeling with CytoTag non-magnetics nanobeads, incubation.
Addition of CytoPull magnetic nanobeads, clustering reagent, CTC enrichment, fluorescent staining, incubation, washes
Release of clustering nanobeads, addition of CytoHandle nanobeads, transfer to imaging cuvette, incubation in magnetic holder
Release of clustering nanobeads, wash, transfer to optical plate, cell fixation to bottom of wells using proprietary protocol
High content screening scan optimized for resolution and multiplex requirements of different assays.
Automated and semi-automated image analysis. Morphology and fluorescent stains used to determine CTC presence. Scoring of analyte stain intensities. Texture image analysis of CTC nuclei to score tumor burden.